Prospective Sorption Matrices for Antitoxic Cholera Enterosorbent Constructing

Abstract

Demonstrated is the possibility of constructing of oral anti-cholera preparations based on sorption matrices with immobilized specific ligand. Various enterosorbents (activated charcoal, polysorb, polyfepan, and chitosan) have been used as sorption matrices, cholera antitoxic immunoglobulins (ATIg) – as specific ligands. Toxin-neutralizing activity of the ATIg immobilized on polysorb has been observed in vivo in 1:64000 dilutions and that of ATIg immobilized on activated charcoal, polyfepan, and chitosan – in 1:32000. Their specific activity in vitro has been defined in dot-blot immunoassay in 1:10000 and 1:5000 dilutions, respectively. High toxin-neutralizing activity, as well as specific one has proved the suitability of all tested matrices for antitoxic cholera enterosorbents constructing. However, polysorb and chitosan are accepted to be the most prospective matrices in view of the studied characteristics and properties. To stabilize the properties of the obtained experimental prototype sorbents, AT/Ig-polysorb and AT/Ig-chitosan, applied has been the method of liophilisation with glycocol stabilizer. Dried samples retain their original biological properties.

Keywords

• энтеросорбенты
• холерный токсин
• сорбционные матрицы
• антитоксические иммуноглобулины
• enterosorbents
• cholera toxin
• sorption matrices
• antitoxic immunoglobulins