Phylogenetic analysis of CPV isolate of Manipur

Abstract

This study was undertaken to determine the prevalent strain and epidemiological pattern of CPV isolates from Manipur. PCR was done with type-specific primers sets pCPV-2, pCPV-2ab and pCPV-2b. Out of 34 samples, 18 CPV isolates were obtained, all of which belong to CPV- 2b. This is the first report of strain characterization of CPV isolates from Manipur (Eastern India) so it was further confirmed by sequencing of the cloned PCR product of 427 bp size. GeneBank accession no. EU118267 was assigned to the sequence. Comparative analysis of the sequence has shown to have cent percent homology with CPV-Japan 97– 008, CPV Africa-3 and CPV-Polish isolates.

Keywords

• Analysis of Sequence
• Canine parvovirus
• Manipur
• Molecular characterization