Comparison of osteoblast cells growth in flat and long bones in mice and rabbits

Abstract

Introduction: Several in vitro studies have shown the effect of external stimulus on acceleration of long bone fracture repair in experimental animals. The aim of this study was to determine feasibility and creation of appropriate conditions for proliferation of the bone cells, in flat and long bones of mice and rabbits . Materials and Methods: Mice and rabbits flat and long bones were cultured in 12 plates. At 14th day of culture, they reached an appropriate level of cell growth. Then specific staining methods were used to study the pattern of cell growth. Results: The results showed a significant differences (P<0.05) in the number of proliferated flat bone (Calvaria) and long bone (Tibia) of rabbits in the first, second and third weeks of cell cultures. The highest rate of growth was observed in the third week in both bones. Similar differences were found in mice. The highest rate of growth in calvaria and tibial bones of mice were found in the second and third weeks, respectively. Conclusion: It seems that growth and proliferation rate of flat bones in mice and rabbits spread more quickly than their lone bones. Thus, this point should be taken into account in the in vitro study of repair pattern of bone tissue.

Keywords

• Cell culture
• Long bone
• Flat bone
• Mice
• Rabbit