The comprehensive transcriptome of human ductus arteriosus smooth muscle cells (hDASMC)
Rachel E.T. Bentley,
Charles C.T. Hindmarch,
Kimberly J. Dunham-Snary,
Brooke Snetsinger,
Jeffrey D. Mewburn,
Arthur Thébaud,
Patricia D.A. Lima,
Bernard Thébaud,
Stephen L. Archer
Affiliations
Rachel E.T. Bentley
Department of Medicine, Queen's University, Kingston, Ontario, Canada
Charles C.T. Hindmarch
Department of Medicine, Queen's University, Kingston, Ontario, Canada; QCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Canada
Kimberly J. Dunham-Snary
Department of Medicine, Queen's University, Kingston, Ontario, Canada; Department of Biomedical and Molecular Science, Queen's University, Canada
Brooke Snetsinger
QCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Canada
Jeffrey D. Mewburn
Department of Biomedical and Molecular Science, Queen's University, Canada
Arthur Thébaud
Department of Kinesiology and Health Studies, Queen's University, Canada
Patricia D.A. Lima
QCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Canada
Bernard Thébaud
Ottawa Hospital Research Institute, Regenerative Medicine Program, Ottawa, Ontario, Canada; Department of Pediatrics, Division of Neonatology, Children's Hospital of Eastern Ontario, Ottawa, Ontario, Canada; Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa, Ontario, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
Stephen L. Archer
Department of Medicine, Queen's University, Kingston, Ontario, Canada; QCPU, Queen's Cardiopulmonary Unit, Translational Institute of Medicine (TIME), Department of Medicine, Queen's University, Canada; Corresponding author(s).
The Ductus Arteriosus (DA) is a fetal vessel that connects the aorta to the pulmonary artery ensuring that placental oxygenated blood is diverted from the lungs to the systemic circulation. Following exposure to oxygen (O2), in the first few days of life, the DA responds with a functional closure that is followed by anatomical closure. Here, we study human DA smooth muscle cells (DASMC) taken from 10 term infants during congenital heart surgery. Purification of these cells using flow cytometry ensured a pure population of DASMCs, which we confirmed as responsive to O2. An oxygen-induced increase in intracellular calcium of 18.1%±4.4% and SMC constriction (-27%±1.5% shortening) occurred in all cell lines within five minutes. These cells were maintained in either hypoxia (2.5% O2), mimicking in utero conditions or in normoxia (19% O2) mimicking neonate conditions. We then used 3’ RNAsequencing to identify the transcriptome of DASMCs in each condition [1]. In this paper, we present the full differentially regulated gene list from this experiment.
