TIMP1 Indicates Poor Prognosis of Renal Cell Carcinoma and Accelerates Tumorigenesis via EMT Signaling Pathway

Yi Shou; Yi Shou; Yuenan Liu; Yuenan Liu; Jiaju Xu; Jiaju Xu; Jingchong Liu; Jingchong Liu; Tianbo Xu; Tianbo Xu; Junwei Tong; Junwei Tong; Lilong Liu; Lilong Liu; Yaxin Hou; Yaxin Hou; Di Liu; Di Liu; Hongmei Yang; Hongmei Yang; Gong Cheng; Gong Cheng; Xiaoping Zhang; Xiaoping Zhang

doi:10.3389/fgene.2022.648134

Frontiers in Genetics (Feb 2022)

TIMP1 Indicates Poor Prognosis of Renal Cell Carcinoma and Accelerates Tumorigenesis via EMT Signaling Pathway

Yi Shou,
Yi Shou,
Yuenan Liu,
Yuenan Liu,
Jiaju Xu,
Jiaju Xu,
Jingchong Liu,
Jingchong Liu,
Tianbo Xu,
Tianbo Xu,
Junwei Tong,
Junwei Tong,
Lilong Liu,
Lilong Liu,
Yaxin Hou,
Yaxin Hou,
Di Liu,
Di Liu,
Hongmei Yang,
Hongmei Yang,
Gong Cheng,
Gong Cheng,
Xiaoping Zhang,
Xiaoping Zhang

Affiliations

Yi Shou: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Yi Shou: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Yuenan Liu: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Yuenan Liu: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Jiaju Xu: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Jiaju Xu: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Jingchong Liu: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Jingchong Liu: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Tianbo Xu: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Tianbo Xu: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Junwei Tong: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Junwei Tong: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Lilong Liu: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Lilong Liu: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Yaxin Hou: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Yaxin Hou: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Di Liu: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Di Liu: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Hongmei Yang: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Hongmei Yang: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Gong Cheng: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Gong Cheng: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Xiaoping Zhang: Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Xiaoping Zhang: Institute of Urologic Surgery, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

DOI: https://doi.org/10.3389/fgene.2022.648134
Journal volume & issue: Vol. 13

Abstract

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Renal cell carcinoma (RCC) is one of the most common malignancies in the urinary system. The mortality of advanced RCC remains high despite advances in systemic therapy of RCC. Considering the misdiagnosis of early-stage RCC, the identification of effective biomarkers is of great importance. Tissue inhibitor matrix metalloproteinase 1 (TIMP1), which belongs to TIMP gene family, is a natural inhibitor of the matrix metalloproteinases (MMPs). In this study, we found TIMP1 was significantly up-regulated in cell lines and RCC tissues. Kaplan-Meier analysis revealed that high expression of TIMP1 indicated a poor prognosis. Multivariate analysis further indicated that TIMP1 overexpression was an independent prognostic factor of RCC patients. Furthermore, knockdown of TIMP1 in vitro suppressed the proliferation, migration, and invasion of RCC cells, while upregulating TIMP1 accelerated the proliferation, migration, and invasion of RCC cells. In addition, we also found that TIMP1 prompted the progression of RCC via epithelial-to-mesenchymal transition (EMT) signaling pathway. In conclusion, the present results suggested that TIMP1 indicated poor prognosis of renal cell carcinoma and could serve as a potential diagnostic and prognostic biomarker for RCC.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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