Current Issues in Molecular Biology (Dec 2023)

DNA Methylation of the IL-17A Gene Promoter Is Associated with Subclinical Atherosclerosis and Coronary Artery Disease: The Genetics of Atherosclerotic Disease Mexican Study

  • Nonanzit Pérez-Hernández,
  • Rosalinda Posadas-Sánchez,
  • Gilberto Vargas-Alarcón,
  • Óscar Pérez-Méndez,
  • María Luna-Luna,
  • José Manuel Rodríguez-Pérez

DOI
https://doi.org/10.3390/cimb45120610
Journal volume & issue
Vol. 45, no. 12
pp. 9768 – 9777

Abstract

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The interleukin-17 (IL-17) has a crucial role during inflammation and has been associated with cardiovascular diseases, but its role in epigenetics is still poorly understood. Therefore, the aim of this study was to evaluate the DNA methylation status of the IL-17A gene promoter to establish whether it may represent a risk factor for subclinical atherosclerosis (SA) or clinical coronary artery disease (CAD). We included 38 patients with premature CAD (pCAD), 48 individuals with SA, and 43 healthy controls. Methylation in the CpG region of the IL-17A gene promoter was assessed via methylation-specific polymerase chain reaction (MSP). Individuals with SA showed increased methylation levels compared to healthy controls and pCAD patients, with p p IL-17A gene promoter DNA represent a risk for symptomatic pCAD when compared with SA patients (OR = 0.16, 95% CI = 0.06–0.41, p IL-17A gene promoter is a risk factor for SA but may be a protection factor for progression from SA to symptomatic CAD.

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