Evaluation of BIRC6 Expression in Oral Squamous Cell Carcinoma, Epithelial Dysplasia, Lichen Planus with and without Dysplasia, and Hyperkeratosis
Fateme Eskandari,
Alireza Razavian,
Razieh Zare,
Shayan Ejlali,
Alireza Razmahang,
Milad Zanjani,
Seyedeh Sara Aghili,
Mohammad Amin Mahdiyar,
Hossein Mofidi,
Kamyar Abbasi,
Ashkan Badkoobeh,
Nafiseh Shamloo,
Lotfollah Kamali Hakim,
Ahmed Hussain,
Hamid Tebyaniyan
Affiliations
Fateme Eskandari
School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Alireza Razavian
Department of Endodontics, Semnan Dental School, Semnan University of Medical Sciences, Semnan 35147-99442, Iran
Razieh Zare
Oral and Maxillofacial Research Center, Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Shayan Ejlali
School of Dentistry, Borujerd Branch, Islamic Azad University, Borujerd 69151-36335, Iran
Alireza Razmahang
Molecular Pathology Research Center, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Milad Zanjani
Department of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Seyedeh Sara Aghili
School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Mohammad Amin Mahdiyar
Department of Orthopedics, School of Medicine, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Hossein Mofidi
Department of Endodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-14336, Iran
Kamyar Abbasi
Department of Prosthodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran 19839-69411, Iran
Ashkan Badkoobeh
Department of Oral and Maxillofacial Surgery, School of Dentistry, Qom University of Medical Sciences, Qom 37136-49373, Iran
Nafiseh Shamloo
Department of Oral and Maxillofacial Pathology, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran 19839-69411, Iran
Lotfollah Kamali Hakim
Independent Researcher, Tehran 16738-48110, Iran
Ahmed Hussain
School of Dentistry, Edmonton Clinic Health Academy, University of Alberta, Edmonton, AB T6G 1C9, Canada
Hamid Tebyaniyan
Department of Science and Research, Islimic Azade University, Tehran 15847-15414, Iran
Background: BIRC6, regarded as the pivotal member of the inhibitor of the apoptosis (IAP) family, has been linked to the development of different types of cancer in humans. The objective of this study was to examine the expression of BIRC6 in various oral conditions, including OLP with dysplasia (OLPD), hyperkeratosis (HK), OLP, epithelial dysplasia (ED), and oral squamous cell carcinoma (OSCC), to investigate its potential involvement in the development of OSCC and the pathogenesis and malignant transformation of OLP, which is known as a precancerous condition. Methods: In this retrospective cross-sectional study, 99 cases, consisting of 19 cases of OSCC, 21 cases of ED, 23 cases of OLP, 20 cases of OLPD, and 16 cases of HK as the control group, were investigated regarding BIRC6 expression by immunohistochemical staining. After that, the immunohistochemical expression of BIRC6 in the epithelial compartment was analyzed. Statistical analysis was performed to investigate the relationship between the expression of BIRC6 and clinicopathological variables. The statistical analysis of the data involved the use of one-way ANOVA, post hoc Tukey, Kruskal–Wallis, Chi-square, Spearman’s correlation, and Mann–Whitney tests. The significance level was set at p p = 0.00). The average total staining score was remarkably greater in OSCC and dysplastic lesions compared with HK (p = 0.00, p = 0.00). Conclusions: While the current study suggested that BIRC6 may play a role in the tumorigenesis of OSCC, its role in the malignant transformation of OLP has yet to be definitively established.
