Nature Communications (Jun 2022)
Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children
- Felix Dewald,
- Isabelle Suárez,
- Ronja Johnen,
- Jan Grossbach,
- Roberto Moran-Tovar,
- Gertrud Steger,
- Alexander Joachim,
- Gibran Horemheb Rubio,
- Mira Fries,
- Florian Behr,
- Joao Kley,
- Andreas Lingnau,
- Alina Kretschmer,
- Carina Gude,
- Guadelupe Baeza-Flores,
- David Laveaga del Valle,
- Alberto Roblero-Hernandez,
- Jesus Magana-Cerino,
- Adriana Torres Hernandez,
- Jesus Ruiz-Quinones,
- Konstantin Schega,
- Viktoria Linne,
- Lena Junker,
- Marie Wunsch,
- Eva Heger,
- Elena Knops,
- Veronica Di Cristanziano,
- Meike Meyer,
- Christoph Hünseler,
- Lutz T. Weber,
- Jan-Christoffer Lüers,
- Gustav Quade,
- Hilmar Wisplinghoff,
- Carsten Tiemann,
- Rainer Zotz,
- Hassan Jomaa,
- Arthur Pranada,
- Ileana Herzum,
- Paul Cullen,
- Franz-Josef Schmitz,
- Paul Philipsen,
- Georg Kirchner,
- Cornelius Knabbe,
- Martin Hellmich,
- Michael Buess,
- Anna Wolff,
- Annelene Kossow,
- Johannes Niessen,
- Sebastian Jeworutzki,
- Jörg-Peter Schräpler,
- Michael Lässig,
- Jörg Dötsch,
- Gerd Fätkenheuer,
- Rolf Kaiser,
- Andreas Beyer,
- Jan Rybniker,
- Florian Klein
Affiliations
- Felix Dewald
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Isabelle Suárez
- Center for Molecular Medicine Cologne, University of Cologne
- Ronja Johnen
- CECAD Research center, University of Cologne
- Jan Grossbach
- CECAD Research center, University of Cologne
- Roberto Moran-Tovar
- Institute for Biological Physics, University of Cologne
- Gertrud Steger
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Alexander Joachim
- Department of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Gibran Horemheb Rubio
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Mira Fries
- Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Florian Behr
- Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Joao Kley
- Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Andreas Lingnau
- Ministry of Schools and Education of North Rhine-Westphalia
- Alina Kretschmer
- Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Carina Gude
- CECAD Research center, University of Cologne
- Guadelupe Baeza-Flores
- Centro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús
- David Laveaga del Valle
- Centro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús
- Alberto Roblero-Hernandez
- Centro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús
- Jesus Magana-Cerino
- Centro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús
- Adriana Torres Hernandez
- Bioclilab SA de CV
- Jesus Ruiz-Quinones
- Centro de Investigación en Enfermedades Tropicales y Emergentes, Hospital Regional de Alta Especialidad, Dr. Juan Graham Casasús
- Konstantin Schega
- Health department of Cologne
- Viktoria Linne
- Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Lena Junker
- Department I of Internal Medicine, Division of Infectious Diseases, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Marie Wunsch
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Eva Heger
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Elena Knops
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Veronica Di Cristanziano
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Meike Meyer
- Department of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Christoph Hünseler
- Department of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Lutz T. Weber
- Department of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Jan-Christoffer Lüers
- Department of Otorhinolaryngology, Head and Neck Surgery, Faculty of Medicine, University of Cologne
- Gustav Quade
- MVZ Labor Dr. Quade & Kollegen GmbH
- Hilmar Wisplinghoff
- Labor Dr. Wisplinghoff
- Carsten Tiemann
- Labor Krone
- Rainer Zotz
- Institute for Laboratory Medicine ZotzKlimas
- Hassan Jomaa
- Synlab
- Arthur Pranada
- Medizinisches Versorgungszentrum Dr. Eberhard & Partner
- Ileana Herzum
- Medizinische Laboratorien Düsseldorf
- Paul Cullen
- MVZ Labor Münster
- Franz-Josef Schmitz
- Mühlenkreiskliniken
- Paul Philipsen
- Labor Mönchengladbach MVZ Dr. Stein und Kollegen
- Georg Kirchner
- Eurofins Laborbetriebsgesellschaft Gelsenkirchen GmbH & Eurofins MVZ Medizinisches Labor Gelsenkirchen GmbH
- Cornelius Knabbe
- Heart- and Diabetes Center NRW, Medical Faculty, Ruhr-University Bochum, Institute for Laboratory and Transfusion Medicine
- Martin Hellmich
- Institute of Medical Statistics and Computational Biology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Michael Buess
- Health department of Cologne
- Anna Wolff
- Health department of Cologne
- Annelene Kossow
- Health department of Cologne
- Johannes Niessen
- Health department of Cologne
- Sebastian Jeworutzki
- Faculty of Social Science, Ruhr-University Bochum
- Jörg-Peter Schräpler
- Faculty of Social Science, Ruhr-University Bochum
- Michael Lässig
- Institute for Biological Physics, University of Cologne
- Jörg Dötsch
- Department of Pediatrics, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Gerd Fätkenheuer
- Center for Molecular Medicine Cologne, University of Cologne
- Rolf Kaiser
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- Andreas Beyer
- CECAD Research center, University of Cologne
- Jan Rybniker
- Center for Molecular Medicine Cologne, University of Cologne
- Florian Klein
- Institute of Virology, Faculty of Medicine, University Hospital Cologne, University of Cologne
- DOI
- https://doi.org/10.1038/s41467-022-30664-2
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 11
Abstract
Dewald et al. combine a non-invasive sampling approach (Lolli-Test) with an RT qPCR-pool testing strategy to screen for SARS-CoV-2 infections in children and use the method for surveillance and infection control in > 4000 school and daycare settings.
