Lineage and sublineage analysis of human papillomavirus type 58 in iranian women

Abstract

Abstract Background Variant analysis of distinct HPV types is important from different aspects including epidemiology, pathogenicity, and evolution. Methods For this reason, the full sequence of the E6 and E7 genes of HPV 58 was examined in 130 HPV 58-infected cervical samples using PCR and sequencing. Results Our results revealed that three lineages A, B, and D were found in this study; among which the B lineage was more common (91.50%). About sublineages, all samples of the B lineage belonged to the B1 sublineage, and samples that were classified as the A and D lineages were found to belong to the A1 (0.77%), A2 (5.38%), A3 (1.50%), and D2 (0.77%) sublineages. No statistically significant differences were found between lineages and stages of disease or amino acid changes (P > 0.05). Conclusion Our results showed that lineage B, sublineage B1, was dominant in Iran. However, more studies with larger sample sizes from different parts of Iran are essential for assessing the pathogenicity risk of HPV 58 lineages in Iranian women with cervical cancer.

Keywords

• Human papillomavirus
• HPV 58
• Lineage
• Sublineage
• Cervical cancer