Probing Individual Particles in Aquatic Suspensions by Simultaneously Measuring Polarized Light Scattering and Fluorescence
Zhihang Xiong,
Hongjian Wang,
Jiajin Li,
Ran Liao,
Haoji Mai,
Caizhong Guan,
Zhiming Guo,
Shangpan Yang,
Yan Chen,
Biwang Liu,
Tong Liu,
Hongyi Li,
Wenzheng Ding,
Yaguang Zeng,
Hui Ma
Affiliations
Zhihang Xiong
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Hongjian Wang
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Jiajin Li
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Ran Liao
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Haoji Mai
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Caizhong Guan
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Zhiming Guo
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Shangpan Yang
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Yan Chen
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Biwang Liu
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Tong Liu
Institute for Ocean Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Hongyi Li
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Wenzheng Ding
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Yaguang Zeng
Department of Photoelectric Technology, Foshan University, Foshan 528000, China
Hui Ma
Guangdong Research Center of Polarization Imaging and Measurement Engineering Technology, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Suspended particles play a significant role in aquatic systems. However, existing methods to probe suspended particles have several limitations. In this paper, we present a portable prototype to in situ probe individual particles in aquatic suspensions by simultaneously measuring polarized light scattering and fluorescence, aiming to obtain an effective classification of microplastics and microalgae. Results show that the obtained classification accuracy is significantly higher than that for either of these two methods. The setup also successfully measures submicron particles and discriminates two species of Synechococcus. Our study demonstrates the feasibility of simultaneously measuring polarized light scattering and fluorescence, and the promising capability of our method for further aquatic environmental monitoring.
