High cell density strategy for poly(3-hydroxybutyrate) production by Cupriavidus necator

Brazilian Journal of Chemical Engineering. 2011;28(4):585-596 DOI 10.1590/S0104-66322011000400004


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Journal Title: Brazilian Journal of Chemical Engineering

ISSN: 0104-6632 (Print); 1678-4383 (Online)

Publisher: Brazilian Society of Chemical Engineering

LCC Subject Category: Technology: Chemical technology: Chemical engineering

Country of publisher: Brazil

Language of fulltext: English

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J. L. Ienczak
L. K. Quines
A. A. de Melo
M. Brandellero
C. R. Mendes
W. Schmidell
G. M. F. Aragão


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Time From Submission to Publication: 12 weeks


Abstract | Full Text

Poly(3-hydroxybutyrate) (P(3HB)) is a carbon and intracellular storage source for different microorganisms and its production can achieve high productivities by means of high cell density cultures. The aim of this study was to propose a high cell density strategy for P(3HB) production by Cupriavidus necator. The exponential growth phase demands an accurate control of the oxygen transfer system in the bioreactor, due to maximum specific growth rate (µXr), and, consequently, a maximum specific oxygen uptake rate (QO2), in addition to significant residual biomass (Xr) growth in high cell density cultures. In this context, this work investigated the strategy for obtaining high cell density, with the inclusion of a linear growth phase for P(3HB) production by C. necator in a fed-batch culture. The linear growth phase was included between the exponential growth phase and the P(3HB) production phase as a strategy to reduce the specific growth rate (µXr) and specific oxygen uptake rate (QO2), with constant residual biomass growth rate (d(V.Xr)/dt = k = constant) and linear increase of biomass. Three strategies of culture were performed. The results showed that a high residual biomass concentration (30 gXr.L-1) can be reached by the inclusion of the linear growth strategy and specific growth rates (µXr) between 0.08 and 0.05 h-1, at the beginning of the production phase, are necessary to attain a high P(3HB) productivity.