In Vitro Culture of Glochidia and Morphological Changes in Juveniles of the Endangered Freshwater Mussel <i>Solenaia oleivora</i>
Xueyan Ma,
Wu Jin,
Guohua Lv,
Wanwen Chen,
Dongpo Xu,
Pao Xu,
Dan Hua,
Haibo Wen
Affiliations
Xueyan Ma
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Wu Jin
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Guohua Lv
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Wanwen Chen
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Dongpo Xu
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Pao Xu
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Dan Hua
Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Haibo Wen
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
The artificial propagation of the endangered Solenaia oleivora, with unknown fish hosts, was performed via in vitro culture with bighead carp, grass carp, common carp, bovine, and rabbit sera. The effects of glochidium density on transformation rates were evaluated, and the development of juveniles that metamorphosed successfully was documented. The control group had a transformation rate of 0 and a contamination rate of 0. No significant differences were found in the transformation and contamination rates of the bighead carp, grass carp, and common carp serum groups, and their transformation rates were significantly higher, and contamination rates were significantly lower than those of the bovine and rabbit serum groups. Moreover, no significant differences were observed in the transformation rates of glochidia (culture density, 2000–5000 glochidia/dish) in contamination-free conditions. Specimen shell length/height increased from 1.08 ± 0.01 on the first day to 3.08 ± 0.29 during the 13th week. On the basis of anterior and posterior growth differences, juvenile growth was divided into the following three phases: the rapid anterior growth period, distinct anterior and posterior idiophase, and rapid posterior growth period. This study not only provides technological support for the artificial propagation of S. oleivora but also lays a foundation for resource recovery.
