Simultaneous detection of OTA and AFB1 in cereals based on bispecific monoclonal antibody using quantum dot nanobead lateral flow immunoassay

Mengyun Wu; Jun Xia; Tingting Liu; Guangjian Xue; Xuechen Fang; Weihua Lai; Juan Peng

doi:10.1080/09540105.2023.2206596

Food and Agricultural Immunology (Dec 2023)

Simultaneous detection of OTA and AFB1 in cereals based on bispecific monoclonal antibody using quantum dot nanobead lateral flow immunoassay

Mengyun Wu,
Jun Xia,
Tingting Liu,
Guangjian Xue,
Xuechen Fang,
Weihua Lai,
Juan Peng

Affiliations

Mengyun Wu: State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Jun Xia: Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang, People’s Republic of China
Tingting Liu: State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Guangjian Xue: State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Xuechen Fang: State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Weihua Lai: State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China
Juan Peng: State Key Laboratory of Food Science and Technology and School of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China

DOI: https://doi.org/10.1080/09540105.2023.2206596
Journal volume & issue: Vol. 34, no. 1
pp. 48 – 66

Abstract

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ABSTRACTSimultaneous contamination of ochratoxin A (OTA) and aflatoxin B1 (AFB1) in cereals are an ongoing global concern. For this reason, a sensitive method for the simultaneous detection of OTA and AFB1 in soybean, corn, and rice is required. Herein, we prepared a bispecific monoclonal antibody (BsMAb) that specifically recognized OTA and AFB1, and subsequently developed a quantum dot nanobead (QB) lateral flow immunoassay (QB-LFIA) based on BsMAb. The half-inhibitory concentrations (IC50) for OTA and AFB1 detection were 0.73 and 0.01 µg/kg in soybean, 1.14 and 0.03 µg/kg in corn, and 1.69 and 0.07 µg/kg in rice, respectively. The average recoveries of OTA and AFB1 were 80.20–130.05% with coefficients of variation below 11%. The performance of the proposed method was compared with that of traditional LFIA, and higher sensitivity was observed. The BsMAb-based QB-LFIA was sensitive and specific for the simultaneous detection of OTA and AFB1 in cereals.

Published in Food and Agricultural Immunology

ISSN: 0954-0105 (Print); 1465-3443 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Agriculture: Agriculture (General); Medicine: Internal medicine: Specialties of internal medicine: Immunologic diseases. Allergy
Website: https://www.tandfonline.com/journals/cfai

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