Analytical Platform for the Study of Metabolic Pathway of Propyl Propane Thiosulfonate (PTSO) from <i>Allium</i> spp.
María García-Nicolás,
Marta Pastor-Belda,
Natalia Campillo,
María Jesús Rodríguez-Sojo,
Antonio Jesús Ruiz-Malagón,
Laura Hidalgo-García,
Paloma Abad,
José Manuel de la Torre,
Enrique Guillamón,
Alberto Baños,
Julio Gálvez,
Pilar Viñas,
Natalia Arroyo-Manzanares
Affiliations
María García-Nicolás
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Marta Pastor-Belda
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Natalia Campillo
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
María Jesús Rodríguez-Sojo
Department of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, Spain
Antonio Jesús Ruiz-Malagón
Department of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, Spain
Laura Hidalgo-García
Department of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, Spain
Paloma Abad
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
José Manuel de la Torre
Department of Inorganic and Organic Chemistry, Campus of Lagunillas, Universidad de Jaén, E-23071 Jaén, Spain
Enrique Guillamón
Department of Microbiology, University of Granada, Fuente Nueva s/n, E-19071 Granada, Spain
Alberto Baños
Department of Microbiology, University of Granada, Fuente Nueva s/n, E-19071 Granada, Spain
Julio Gálvez
Department of Pharmacology, Instituto de Investigación Biosanitaria (ibs. GRANADA), Center for Biomedical Research (CIBM), University of Granada, E-18071 Granada, Spain
Pilar Viñas
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
Natalia Arroyo-Manzanares
Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, E-30100 Murcia, Spain
The present work is focused on the development of an analytical platform to elucidate the metabolic pathway of PTSO from onion, an organosulfur compound well-known for its functional and technological properties and its potential application in animal and human nutrition. This analytical platform consisted of the use of gas chromatography–mass spectrometry (GC-MS) and ultra-high performance liquid chromatography quadrupole with time-of-flight MS (UHPLC-Q-TOF-MS) in order to monitor volatile and non-volatile compounds derived from the PTSO. For the extraction of the compounds of interest, two different sample treatments were developed: liquid–liquid extraction (LLE) and salting-out assisted liquid–liquid extraction (SALLE) for GC–MS and UHPLC-Q-TOF-MS analysis, respectively. Once the analytical platform was optimised and validated, an in vivo study was planned to elucidate PTSO metabolisation, revealing the presence of dipropyl disulfide (DPDS) in liver samples with concentrations between 0.11 and 0.61 µg g−1. The DPDS maximum concentration in the liver was observed at 0.5 h after the intake. DPDS was also present in all plasma samples with concentrations between 2.1 and 2.4 µg mL−1. In regard to PTSO, it was only found in plasma at times above 5 h (0.18 µg mL−1). Both PTSO and DPDS were excreted via urine 24 h after ingestion.
