Nature Communications (May 2019)

Integrating Hi-C and FISH data for modeling of the 3D organization of chromosomes

  • Ahmed Abbas,
  • Xuan He,
  • Jing Niu,
  • Bin Zhou,
  • Guangxiang Zhu,
  • Tszshan Ma,
  • Jiangpeikun Song,
  • Juntao Gao,
  • Michael Q. Zhang,
  • Jianyang Zeng

DOI
https://doi.org/10.1038/s41467-019-10005-6
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 14

Abstract

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Methodological advances have increased our understanding of chromatin structure through chromosome conformation capture techniques and high resolution imaging, but integration of these datasets is challenging. Here the authors propose GEM-FISH, a method for reconstructing the 3D models of chromosomes through systematically integrating both Hi-C and FISH data with the prior biophysical knowledge of a polymer model.