Effects of Heat Stress on Production Indices, Antioxidant Function, Heat Shock Protein and Intestinal Microflora in Quails

Abstract

ABSTRACT Gut microbiota plays an important role in animal health, production and diseases. Little is known about whether heat stress alters the composition of quail gut microbiota; therefore, we studied the effects of heat stress on growth performance, antioxidant functions, heat shock proteins and caecal microbiota. Two groups of 40 (20-day-old) quails were set up, including a control reared at 24 ± 2°C and a heat stress group subjected to heat stress at 36 ± 2°C for 4 h per day for 7 consecutive days. We found that heat stress significantly elevated the relative expression levels of HSP70 and HSP90 mRNA in the thymus, bursa and spleen by real-time fluorescence quantitative PCR assay, indicating successful establishment of the experimental model. Heat stress was found to have an effect on gut microbiota composition. At the genus level, Alistipes were significantly increased in the heat stress group. PICRUSt2 function prediction revealed that most of the KEGG pathways with high temperature-induced abundance differences are metabolism-related. These data indicated that heat stress reduced the production performance of quails by affecting antioxidant functions, as well as the composition and structure of the intestinal microbiota. The results of this study provide technical information for conducting research on heat stress prevention and control techniques in quails.

Keywords

• Antioxidant function
• Heat shock protein
• Heat stress
• Intestinal microbiota
• Quail