Difference of hemaglutinins between wild-type and vaccine measles virus in Indonesia

Abstract

Background Hemaglutinin (H) protein of measles virus is very important in the process of host cell infection. H protein is also able to induce specific antibodies which can neutralize measles virus and block the cell infection. Objective This study aimed to explore the nucleotide and amino acid sequence differences between wild-type measles virus (G2, G3 and D9) with CAM-70, Schwarz and Edmonston-wt vaccine virus. Methods The exctration and amplification of the gene were conducted in the laboratory using biomolecular technology. The gene and protein analysis were conducted using the bioinformatic technology. Results The results showed that the differences in nucleotide sequences were highest between wild-type virus and CAM-70 vaccine virus (76-77 nucleotides), followed by Schwarz (61-64 nucleotides) and Edmonston (60-63 nucleotides). The differences in amino acid sequences were highest between wild-type virus and CAM-70 (24-29 residues), followed by Schwarz (13-20 residues) and Edmonston (12-19 residues). Conclusion The Indonesian wild-type measles virus was genetically closer to Schwarz vaccine virus than CAM-70 vaccine virus, hence the neutralizing antibodies generated by Schwarz vaccine were more specific against Indonesian wild-type virus compared to CAM-70 vaccine.

Keywords

• measles
• wilde-type
• vaccine-type
• hemagglutinin gene
• hemagglutinin protein