European Journal of Histochemistry (Jun 2009)

Bismuth ions are metabolized into autometallographic traceable bismuth-sulphur quantum dots

  • M Stoltenberg,
  • S Juhl,
  • G Danscher

DOI
https://doi.org/10.4081/1012
Journal volume & issue
Vol. 51, no. 1
pp. 53 – 58

Abstract

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Bismuth – sulphur quantum dots can be silver enhanced by autometallography (AMG). In the present study, autometallographic silver enhanced bismuth-sulphur nanocrystals were isolated from unfixed cryo-sections of kidneys and livers of rats exposed to bismuth (Bi207) subnitrate. After being subjected to AMG all the organic material was removed by sonication and enzymatic digestion and the silver enhanced Bi- S quantum dots spun down by an ultracentrifuge and analyzed by scintillation. The analysis showed that the autometallographic technique traces approximately 94% of the total bismuth. This implies that the injected bismuth is ultimately captured in bismuthsulphur quantum dots, i.e., that Bi-S nanocrystals are the end product of bismuth metabolism