Acta Pharmaceutica (Mar 2022)

UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

  • Massadeh Rafeef K.,
  • El-Elimat Tamam,
  • Al-Gharaibeh Mohammad,
  • Tawaha Khaled,
  • Alali Feras Q.

DOI
https://doi.org/10.2478/acph-2022-0001
Journal volume & issue
Vol. 72, no. 1
pp. 97 – 108

Abstract

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The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

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