OncoTargets and Therapy (Jul 2020)

Coronin 3 Promotes the Development of Oncogenic Properties in Glioma Through the Wnt/β-Catenin Signaling Pathway

  • Wang M,
  • Li Q,
  • Yu S,
  • Zhang Z,
  • Qiu P,
  • Zhang Y,
  • Yang W,
  • Xu G,
  • Xu T

Journal volume & issue
Vol. Volume 13
pp. 6661 – 6673

Abstract

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Min Wang,1 Qi Li,1 Shengyuan Yu,1 Zexiang Zhang,2 Peng Qiu,1 Yubao Zhang,1 Wei Yang,1 Guangming Xu,1 Tongjiang Xu1 1Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250014, People’s Republic of China; 2Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250014, People’s Republic of ChinaCorrespondence: Tongjiang XuDepartment of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250014, People’s Republic of ChinaTel +86-531-87938911Email [email protected]: Evidence indicates that the actin-binding protein Coronin 3, which is aberrantly expressed in various cancers, is associated with cancer development and progression. However, little is known about the role of Coronin 3 in glioma tumorigenesis. Here, we aimed to explore the biological function and regulatory mechanism of Coronin 3 in glioblastoma (GBM).Materials and Methods: Coronin 3 level in human GBM clinical samples and cell lines was investigated. The shRNA knockdown strategy was used to assess the tumor characteristics of GBM cell lines. The role of β-catenin in Coronin 3-mediated oncogenic phenotypes was evaluated.Results: Coronin 3 was found to be highly upregulated in glioma cell lines. Furthermore, knockdown of Coronin 3 significantly inhibited the growth of glioma cells both in vivo and in vitro and suppressed the expression of Wnt/β-catenin pathway genes, including β-catenin, Cyclin D1, and c-Myc. Moreover, we demonstrated that Coronin 3 regulates the expression of β-catenin in glioma. Our results revealed that Coronin 3-stimulated tumor growth was β-catenin-dependent.Conclusion: Our study reveals a new molecular mechanism of Coronin 3 in promoting glioma growth and development through regulating the Wnt/β-catenin signaling pathway.Keywords: glioblastoma, Coronin 3, proliferation, oncogenic property, Wnt/β-catenin

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