Nature Communications (Jan 2018)

Comprehensive profiling of the ligand binding landscapes of duplexed aptamer families reveals widespread induced fit

  • Jeffrey D. Munzar,
  • Andy Ng,
  • David Juncker

DOI
https://doi.org/10.1038/s41467-017-02556-3
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 15

Abstract

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Duplexed aptamers are a common biosensor format; however, how complementary strand sequence, length, and position modulate ligand binding is not well understood. Here, the authors introduce ACE-Scan to comprehensively map binding landscapes, uncovering hotspots of enhanced binding by induced fit.