Cancer Management and Research (Nov 2020)
lncRNA SNHG1 Promotes Progression of Cervical Cancer Through miR-195/NEK2 Axis
Abstract
Yuan Yuan Ji,1 Man Meng,2 Ye Miao3 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People’s Republic of China; 2Department of Geriatrics, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People’s Republic of China; 3Department of Neurosurgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, People’s Republic of ChinaCorrespondence: Ye MiaoDepartment of Neurosurgery, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, People’s Republic of ChinaEmail [email protected]: Cervical cancer is a common gynecologic cancer, and no study has been reported on the way through which lncRNA SNHG1, miR-195 and NEK2 jointly affect cervical cancer cells (CCCs), so this paper will explore a new approach to the development of cervical cancer in this respect.Methods: Altogether 72 cervical cancer tissues and 54 adjacent tissues were collected. qPCR was performed to quantify lncRNA SNHG1 and miR-195, whose expression vectors were constructed and then transfected into CCCs, so as to observe their effects on the cells. Western blotting (WB) was carried out to detect protein levels. MTT assay was conducted to detect cell activity. Flow cytometry was performed to detect cell apoptosis. Transwell was carried out to detect cell invasion and migration.Results: The expression of lncRNA SNHG1 up-regulated while that of miR-195 down-regulated in CCCs. lncRNA SNHG1 regulated NEK2 through its targeted binding to miR-195. The down-regulation of lncRNA SNHG1 or the up-regulation of miR-195 led to the decrease of NEK2 and the reduction of cells’ activity, migration and invasion, also resulting in the increase of cell apoptosis. Rescue experiments showed that the down-regulation of miR-195 could offset the cell changes caused by lncRNA SNHG1.Conclusion: lncRNA SNHG1 promotes the progression of cervical cancer through the miR-195/NEK2 axis, so lncRNA SNHG1, miR-195 and NEK2 may have potential values for diagnosing and treating cervical cancer.Keywords: cervical cancer, lncRNA SNGH1, miR-195, NEK2
