Transplant International (Nov 2024)
The impact of the EVLP on the lung microbiome and its inflammatory reaction
- Leandro Grando,
- Marc Boada,
- Rosa Faner,
- Rosa Faner,
- Rosa Faner,
- Rosa Faner,
- Susana Gómez-Ollés,
- Susana Gómez-Ollés,
- Victoria Ruiz,
- Marc Bohils,
- Joaquim Albiol,
- Ramses Marrero,
- Laia Rosell,
- Ivan Salinas,
- Daniel Ruiz,
- Ángel Ruiz,
- Camino Rodríguez-Villar,
- Anna Ureña,
- David Paredes-Zapata,
- Ángela Guirao,
- Gerard Sánchez-Etayo,
- Laureano Molins,
- Néstor Quiroga,
- Aroa Gómez-Brey,
- Xavier Michavila,
- Alberto Sandiumenge,
- Àlvar Agustí,
- Àlvar Agustí,
- Àlvar Agustí,
- Ricard Ramos,
- Ricard Ramos,
- Irene Bello,
- Irene Bello
Affiliations
- Leandro Grando
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Marc Boada
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Rosa Faner
- Universitat de Barcelona, Catedra Salut Respiratoria, Barcelona, Catalunya, Spain
- Rosa Faner
- CIBER Enfermedades Respiratorias (CIBERES), Madrid, Spain
- Rosa Faner
- Institut d’Investigacions BIomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
- Rosa Faner
- Universitat de Barcelona, Biomedicine Department, Barcelona, Catalunya, Spain
- Susana Gómez-Ollés
- CIBER Enfermedades Respiratorias (CIBERES), Madrid, Spain
- Susana Gómez-Ollés
- Department of Pneumology, Vall d’Hebron Institut de Recerca, Barcelona, Spain
- Victoria Ruiz
- Department of Pneumology, Vall d’Hebron Institut de Recerca, Barcelona, Spain
- Marc Bohils
- Department of Donor and Transplant Coordination, Hospital Clínic, Barcelona, Spain
- Joaquim Albiol
- Department of Donor and Transplant Coordination, Hospital Clínic, Barcelona, Spain
- Ramses Marrero
- Department of Anaesthesia and Perioperative Care, Hospital Clínic of Barcelona, Barcelona, Spain
- Laia Rosell
- Surgical Area, Hospital Clínic, Barcelona, Spain
- Ivan Salinas
- Surgical Area, Hospital Clínic, Barcelona, Spain
- Daniel Ruiz
- Surgical Area, Hospital Clínic, Barcelona, Spain
- Ángel Ruiz
- Department of Donor and Transplant Coordination, Hospital Clínic, Barcelona, Spain
- Camino Rodríguez-Villar
- Department of Donor and Transplant Coordination, Hospital Clínic, Barcelona, Spain
- Anna Ureña
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- David Paredes-Zapata
- Department of Donor and Transplant Coordination, Hospital Clínic, Barcelona, Spain
- Ángela Guirao
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Gerard Sánchez-Etayo
- Department of Donor and Transplant Coordination, Hospital Clínic, Barcelona, Spain
- Laureano Molins
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Néstor Quiroga
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Aroa Gómez-Brey
- 0Department of Donor and Transplant Coordination, Vall d’Hebron University Hospital, Barcelona, Spain
- Xavier Michavila
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Alberto Sandiumenge
- 0Department of Donor and Transplant Coordination, Vall d’Hebron University Hospital, Barcelona, Spain
- Àlvar Agustí
- Universitat de Barcelona, Catedra Salut Respiratoria, Barcelona, Catalunya, Spain
- Àlvar Agustí
- CIBER Enfermedades Respiratorias (CIBERES), Madrid, Spain
- Àlvar Agustí
- 1Department of Pneumology, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Ricard Ramos
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Ricard Ramos
- Institut d’Investigacions BIomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
- Irene Bello
- Department of Thoracic Surgery, Respiratory Institute, Hospital Clínic, Barcelona, Spain
- Irene Bello
- Institut d’Investigacions BIomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
- DOI
- https://doi.org/10.3389/ti.2024.12979
- Journal volume & issue
-
Vol. 37
Abstract
The pulmonary microbiome has emerged as a significant factor in respiratory health and diseases. Despite the sterile conditions maintained during ex vivo lung perfusion (EVLP), the use of antibiotics in the perfuse liquid can lead to dynamic changes in the lung microbiome. Here, we present the design of a study that aims to investigate the hypothesis that EVLP alters the lung microbiome and induces tissue inflammation. This pilot, prospective, controlled study will be conducted in two Spanish donor centers and will include seven organ donors after brain death or after controlled cardiac death. After standardized retrieval, the left lung will be preserved in cold storage and the right lung will be perfused with EVLP. Samples from bronchoalveolar lavage, perfusion and preservation solutions, and lung biopsies will be collected from both lungs and changes in lung microbiome and inflammatory response will be compared.
Keywords
