Metaproteomics reveals parallel utilization of colonic mucin glycans and dietary fibers by the human gut microbiota
Grete Raba,
Ana S. Luis,
Hannah Schneider,
Indrek Morell,
Chunsheng Jin,
Signe Adamberg,
Gunnar C. Hansson,
Kaarel Adamberg,
Liisa Arike
Affiliations
Grete Raba
Department of Chemistry and Biotechnology, Tallinn University of Technology, 12618 Tallinn, Estonia; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden; Corresponding author
Ana S. Luis
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden; SciLifeLab, University of Gothenburg, 41390 Gothenburg, Sweden
Hannah Schneider
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden
Indrek Morell
Center of Food and Fermentation Technologies, 12618 Tallinn, Estonia
Chunsheng Jin
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden
Signe Adamberg
Department of Chemistry and Biotechnology, Tallinn University of Technology, 12618 Tallinn, Estonia
Gunnar C. Hansson
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden
Kaarel Adamberg
Department of Chemistry and Biotechnology, Tallinn University of Technology, 12618 Tallinn, Estonia; Center of Food and Fermentation Technologies, 12618 Tallinn, Estonia
Liisa Arike
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden
Summary: A diet lacking dietary fibers promotes the expansion of gut microbiota members that can degrade host glycans, such as those on mucins. The microbial foraging on mucin has been associated with disruptions of the gut-protective mucus layer and colonic inflammation. Yet, it remains unclear how the co-utilization of mucin and dietary fibers affects the microbiota composition and metabolic activity. Here, we used 14 dietary fibers and porcine colonic and gastric mucins to study the dynamics of mucin and dietary fiber utilization by the human fecal microbiota in vitro. Combining metaproteome and metabolites analyses revealed the central role of the Bacteroides genus in the utilization of complex fibers together with mucin while Akkermansia muciniphila was the main utilizer of sole porcine colonic mucin but not gastric mucin. This study gives a broad overview of the colonic environment in response to dietary and host glycan availability.
