Investigation of endocrine response of thyroid and intestinal and adipose tissues due to the addition of Moringa oleifera essential oil in diet for quails exposed to heat stress

Abstract

ABSTRACT In this study, we aimed to determine the effect of Moringa oleifera essential oil (MOEO) supplementation to rations of Japanese quail ( Coturnix coturnix japonica ) grown under heat stress (HS) on some adipokine (visfatin, adiponectin, and chemerin), intestinal (citrulline), 3,5,3′-triiodothyronine (T3), thyroxine (T4), and thyroid-stimulation hormone (TSH) levels in the serum. For this purpose, 320 day-old male quail chicks were used. The study was conducted over 42 days, including a 7-d adaptation period and a 35-d experimental period. Eight trial groups were established, each including 10 quail: CT (control temperature, 25 ℃), MOEO 200, MOEO 400, MOEO 600, HSC (HS control), SMOEO 200, SMOEO 400, and SMOEO 600. Based on the results, a dose-dependent decrease was observed on days 21 and 42 in serum adiponectin and T3 in the stress and stress-free groups compared with the control group due to MOEO supplementation. The lowest decrease was observed in the MOEO 600 mg/kg dose group. In addition, an increase in stressed groups was observed when examining serum citrulline levels, while chemerin levels did not have a statistically significant effect. While the decrease in serum visfatin, T4, and TSH levels on the 21st day did not have a statistically significant effect, a significant effect was determined on the 42nd day. The addition of MOEO at 600 mg/kg to the ration may be beneficial in preventing intestinal damage and inflammation that may occur due to HS.

Keywords

• adiponectin
• chemerin
• citrulline
• Japanese quail
• thyroid hormones
• visfatin