PCR Optimization for Polyketide Synthase (PKS) and Non-Ribosomal Peptide Synthetase (NRPS) Gene Detection in Actinomycetes

Abstract

Actinomycetes are known as a group of antimicrobial-producing bacteria. This is supported by the presence of potential genes in actinomycetes bacteria. These genes include Polyketide Synthase (PKS) and Non-Ribosomal Peptide Synthetase (NRPS). Detection of these genes using PCR requires the optimum annealing temperature so that the detection process runs accurately. The purpose of this study was to determine the appropriate annealing temperature in the detection of PKS I, PKS II and NRPS genes in actinomycetes bacterial isolates. The study was carried out experimentally with varying annealing temperatures of 52°C and 55°C. The results showed that all three genes were detected at 52°C, while at 55°C the PKS I gene bands were faint, and no PKS II and NRPS gene bands were found. Based on the results obtained, a temperature of 52°C is a suitable temperature for the detection of PKS I, II and NRPS genes.