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Leishmanicidal and fungicidal activity of lipases obtained from endophytic fungi extracts.

PLoS ONE. 2018;13(6):e0196796 DOI 10.1371/journal.pone.0196796


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Journal Title: PLoS ONE

ISSN: 1932-6203 (Online)

Publisher: Public Library of Science (PLoS)

LCC Subject Category: Medicine | Science

Country of publisher: United States

Language of fulltext: English

Full-text formats available: PDF, HTML, XML



Daniela Ribeiro Alves

Selene Maia de Morais

Fernanda Tomiotto-Pellissier

Fábio Roger Vasconcelos

Francisco das Chagas Oliveira Freire

Isaac Neto Goes da Silva

Alan Henrique Depieri Cataneo

Milena Menegazzo Miranda-Sapla

Gustavo Adolfo Saavedra Pinto

Ivete Conchon-Costa

Arlindo de Alencar Araripe Noronha

Wander Rogério Pavanelli


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Time From Submission to Publication: 24 weeks


Abstract | Full Text

This work describes the production of lipases from endophytic fungi: Vermisporium-like, Emericella nidulans, Dichotomophtora portulacae and D. boerhaaviae and the biological activity against the dermatophyte fungi Malassezia sp and Microsporum canis and the parasite Leishmania amazonensis. All fungal enzymes extract showed lipolysis action in the media that contains long carbon chain lipids. The proteomic analysis of lipases exhibits several molecules mostly ranging in size from 220 to 20 kDa, with clear differences in protein profile's yield. All fungal enzymes were competent to eliminate promastigote forms of Leishmania amazonensis at 5 mg.mL-1. The antileishmanial activity of lipases from Vermisporium-like, E. nidulans, D. portulacae and D. boerhaaviae in amastigote forms, promoted the reduction in viability of 78.88, 39.65, 63.17 and 98.13%, with selectivity index of 19.56, 30.68, 18.09 and 20.99. In relation to antifungal activity, Dichothomophtora enzymes demonstrate best action with MFC of 14.65 μg.mL-1 against Malassezia sp and Microsporum canis, respectively. These results allow us to infer that lipases from entophytic fungi displays activity against dermatophyte fungi (Malassezia sp. and Microsporum canis) as well as Leishmania.