PLoS ONE (2018-01-01)

Leishmanicidal and fungicidal activity of lipases obtained from endophytic fungi extracts.

  • Daniela Ribeiro Alves,
  • Selene Maia de Morais,
  • Fernanda Tomiotto-Pellissier,
  • Fábio Roger Vasconcelos,
  • Francisco das Chagas Oliveira Freire,
  • Isaac Neto Goes da Silva,
  • Alan Henrique Depieri Cataneo,
  • Milena Menegazzo Miranda-Sapla,
  • Gustavo Adolfo Saavedra Pinto,
  • Ivete Conchon-Costa,
  • Arlindo de Alencar Araripe Noronha,
  • Wander Rogério Pavanelli

DOI
https://doi.org/10.1371/journal.pone.0196796
Journal volume & issue
Vol. 13, no. 6
p. e0196796

Abstract

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This work describes the production of lipases from endophytic fungi: Vermisporium-like, Emericella nidulans, Dichotomophtora portulacae and D. boerhaaviae and the biological activity against the dermatophyte fungi Malassezia sp and Microsporum canis and the parasite Leishmania amazonensis. All fungal enzymes extract showed lipolysis action in the media that contains long carbon chain lipids. The proteomic analysis of lipases exhibits several molecules mostly ranging in size from 220 to 20 kDa, with clear differences in protein profile's yield. All fungal enzymes were competent to eliminate promastigote forms of Leishmania amazonensis at 5 mg.mL-1. The antileishmanial activity of lipases from Vermisporium-like, E. nidulans, D. portulacae and D. boerhaaviae in amastigote forms, promoted the reduction in viability of 78.88, 39.65, 63.17 and 98.13%, with selectivity index of 19.56, 30.68, 18.09 and 20.99. In relation to antifungal activity, Dichothomophtora enzymes demonstrate best action with MFC of 14.65 μg.mL-1 against Malassezia sp and Microsporum canis, respectively. These results allow us to infer that lipases from entophytic fungi displays activity against dermatophyte fungi (Malassezia sp. and Microsporum canis) as well as Leishmania.