Cell Journal (Nov 2023)

Association of MGLL Intronic C>T Single Nucleotide Polymorphism (rs782440) with Borderline Personality Disorder: A Case-Control Study

  • Nazanin Hatami Bavarsad,
  • Leila Jahangard,
  • Masood Saidijam,
  • Seyed Asaad Karimi,
  • Ali Reza Soltanian,
  • Elahe Shahriari,
  • Saeid Afshar,
  • Abdolrahman Sarihi

DOI
https://doi.org/10.22074/cellj.2023.2004323.1321
Journal volume & issue
Vol. 25, no. 11
pp. 783 – 789

Abstract

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Objective: From the perspective of etiology, borderline personality disorder (BPD) is a multifactorial and complexdisorder, hence our understanding about the molecular basis and signaling of this disorder is extremely limited.The purpose of this study was evaluating the relationship between BPD and the Monoacylglycerol lipase (MGLL)polymorphism rs782440 in the population of Hamadan, Iran.Materials and Methods: In this case-control study, 106 participants including 53 patients with BPD and 53healthy control subjects were selected by psychiatrists in the Department of Psychiatry at Farshchian SinaHospital in Hamadan. The BPD patients were selected based on the Diagnostic and Statistical Manual of MentalDisorders (DSM-5) form for diagnosing BPD patients. For genotyping, polymerase chain reaction (PCR) wasused to amplify the desired region including the MGLL intronic C>T single nucleotide polymorphism (SNP)(rs782440) and afterward the amplicon was sequenced using the Sanger sequencing method. To determine thegenotype of these patients, their sequences were aligned with the reference sequence of MGLL through the CLCgenomic workbench software.Results: The results indicated that the frequency of TT in comparison to the CC genotype was significantly different(P=0.003) and the risk of BPD in change from the TT genotype to CC genotype was increased by 6.679%. Regardingthe frequency of allele in this group, no significant difference was observed.Conclusion: This paper, has studied and reports for the first time, the association between MGLL SNP (rs782440) withBPD. The findings of the current research revealed that the TT genotype increases the risk of BPD compared to the CCgenotype. Considering the lack of a suitable diagnostic biomarker for BPD, using this potential biomarker in the nearfuture can be promising.

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