Nature Communications (May 2021)
Multiplexed detection of SARS-CoV-2 and other respiratory infections in high throughput by SARSeq
- Ramesh Yelagandula,
- Aleksandr Bykov,
- Alexander Vogt,
- Robert Heinen,
- Ezgi Özkan,
- Marcus Martin Strobl,
- Juliane Christina Baar,
- Kristina Uzunova,
- Bence Hajdusits,
- Darja Kordic,
- Erna Suljic,
- Amina Kurtovic-Kozaric,
- Sebija Izetbegovic,
- Justine Schaeffer,
- Peter Hufnagl,
- Alexander Zoufaly,
- Tamara Seitz,
- VCDI,
- Manuela Födinger,
- Franz Allerberger,
- Alexander Stark,
- Luisa Cochella,
- Ulrich Elling
Affiliations
- Ramesh Yelagandula
- Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC)
- Aleksandr Bykov
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Alexander Vogt
- Vienna Biocenter Core Facilities GmbH (VBCF)
- Robert Heinen
- Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC)
- Ezgi Özkan
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Marcus Martin Strobl
- Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC)
- Juliane Christina Baar
- Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC)
- Kristina Uzunova
- Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC)
- Bence Hajdusits
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Darja Kordic
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Erna Suljic
- Clinical Center of the University of Sarajevo
- Amina Kurtovic-Kozaric
- Clinical Center of the University of Sarajevo
- Sebija Izetbegovic
- Clinical Center of the University of Sarajevo
- Justine Schaeffer
- Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES)
- Peter Hufnagl
- Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES)
- Alexander Zoufaly
- Department for Infectious Diseases, Clinic Favoriten
- Tamara Seitz
- Department for Infectious Diseases, Clinic Favoriten
- VCDI
- Manuela Födinger
- Faculty of Medicine, Sigmund Freud Private University
- Franz Allerberger
- Österreichische Agentur für Gesundheit und Ernährungssicherheit (AGES)
- Alexander Stark
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Luisa Cochella
- Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC)
- Ulrich Elling
- Institute of Molecular Biotechnology of the Austrian Academy of Science (IMBA), Vienna BioCenter (VBC)
- DOI
- https://doi.org/10.1038/s41467-021-22664-5
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 17
Abstract
Massively parallel but cost-effective testing is essential to monitor the spread of pathogenic agents. Here the authors present SARSseq, which uses a dual indexing strategy in a multiplexed RT-PCR reaction to diagnose SARS-CoV-2 at scale.
