Journal of Islamic International Medical College (Jun 2019)
Nephroprotective Effect of Aqueous Extract of Carica Papaya Seeds
Abstract
Objective: To determine the nephroprotective effect of aqueous extract of Carica papaya seeds in Aminoglycoside induced acute nephrotoxicity in rats. Study Design: Quasi Experimental study. Place and Duration of Study: The study was conducted from April 2016 to March 2017 at “Department of Pharmacology and Therapeutics” and “Multidisciplinary Laboratory of Islamic International Medical College, Riphah International University”, Islamabad in collaboration with National Institute of Health (NIH), Islamabad. Materials and Methods: Thirty Sprague Dawley rats were divided into 3 groups i.e., A, B and C with 10 rats in each group. Group B and C were given Aminoglycoside; Gentamycin in 80 mg/kg) via intraperitoneal route once th daily for 5 consecutive days to induce acute nephrotoxicity. At day 6 , nephrotoxicity was confirmed by measuring serum urea and creatinine. Aqueous extract of Carica papaya seeds (1000 mg/kg) was started once daily through oral route in group C for 5 consecutive days. All animals were given standard diet pellets manufactured at NIH. Results: Mean serum urea and creatinine for Group A (Control Group) at day 0 was 24.70 mg/dL 5.16 and 0.750 mg/dL 0.1958 respectively. Mean serum urea and creatinine for Group B (Disease Control Group) was 78.60 mg/dL 3.921 and 1.920 0.1229 at day 6th. This suggested induction of nephrotoxicity by gentamycin. Mean th serum urea and creatinine for Group C (Aqueous Extract Treated Group) at 11 day was 50.60 mg/dL 5.910 and 1.380 mg/dL 0.1932 after 5 days treatment with aqueous extract of Carica papaya seeds. Conclusion: Aqueous extract of Carica papaya seeds has significant nephroprotective effects on aminoglycoside induced acute nephrotoxicity in rats.
