Animal Cells and Systems (Dec 2024)

Effect of RNF113A deficiency on oxidative stress-induced NRF2 pathway

  • Namjoon Cho,
  • Yong-Eun Kim,
  • Yunkyeong Lee,
  • Dong Wook Choi,
  • Chungoo Park,
  • Jung-Hwan Kim,
  • Keun Il Kim,
  • Kee K. Kim

DOI
https://doi.org/10.1080/19768354.2024.2349758
Journal volume & issue
Vol. 28, no. 1
pp. 261 – 271

Abstract

Read online

ABSTRACTThe ring finger protein 113A (RNF113A) serves as an E3 ubiquitin ligase and a subunit of the spliceosome. Mutations in the RNF113A gene are associated with X-linked trichothiodystrophy (TTD). However, the cellular roles of RNF113A remain largely unknown. In this study, we performed transcriptome profiling of RNF113A knockout (KO) HeLa cells using RNA sequencing and revealed the upregulation of NRF2 pathway-associated genes. Further analysis confirmed that the KO of RNF113A promotes nuclear localization of the NRF2 protein and elevates the mRNA levels of NRF2 target genes. RNF113A KO cells showed high levels of intracellular reactive oxygen species (ROS) and decreased resistance to cell death following H2O2 treatment. Additionally, RNF113A KO cells more sensitively formed stress granules (SGs) under arsenite-induced oxidative stress. Moreover, RNF113A KO cells exhibited a decrease in glutathione levels, which could be attributed to a reduction in GLUT1 expression levels, leading to decreased glucose uptake reactions and lower intracellular glucose levels. These alterations potentially caused a reduction in ROS scavenging activity. Taken together, our findings suggest that the loss of RNF113A promotes oxidative stress-mediated activation of the NRF2 pathway, providing novel insights into RNF113A-associated human diseases.

Keywords