Antinociceptive Effect of Tephrosia sinapou Extract in the Acetic Acid, Phenyl-p-benzoquinone, Formalin, and Complete Freund’s Adjuvant Models of Overt Pain-Like Behavior in Mice
Renata M. Martinez,
Ana C. Zarpelon,
Talita P. Domiciano,
Sandra R. Georgetti,
Marcela M. Baracat,
Isabel C. Moreira,
Cesar C. Andrei,
Waldiceu A. Verri,
Rubia Casagrande
Affiliations
Renata M. Martinez
Department of Pharmaceutical Sciences, Health Science Centre, University Hospital, Londrina State University, Londrina, PR, Brazil
Ana C. Zarpelon
Departamento de Patologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil
Talita P. Domiciano
Departamento de Patologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil
Sandra R. Georgetti
Department of Pharmaceutical Sciences, Health Science Centre, University Hospital, Londrina State University, Londrina, PR, Brazil
Marcela M. Baracat
Department of Pharmaceutical Sciences, Health Science Centre, University Hospital, Londrina State University, Londrina, PR, Brazil
Isabel C. Moreira
Universidade Tecnológica Federal do Paraná, Londrina, PR, Brazil
Cesar C. Andrei
Laboratório de Pesquisa em Moléculas Bioativas, Departamento de Química, Universidade Estadual de Londrina, Londrina, PR, Brazil
Waldiceu A. Verri
Departamento de Patologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil
Rubia Casagrande
Department of Pharmaceutical Sciences, Health Science Centre, University Hospital, Londrina State University, Londrina, PR, Brazil
Tephrosia toxicaria, which is currently known as Tephrosia sinapou (Buc’hoz) A. Chev. (Fabaceae), is a source of compounds such as flavonoids. T. sinapou has been used in Amazonian countries traditional medicine to alleviate pain and inflammation. The purpose of this study was to evaluate the analgesic effects of T. sinapou ethyl acetate extract in overt pain-like behavior models in mice by using writhing response and flinching/licking tests. We demonstrated in this study that T. sinapou extract inhibited, in a dose (1–100 mg/kg) dependent manner, acetic acid- and phenyl-p-benzoquinone- (PBQ-) induced writhing response. Furthermore, it was active via intraperitoneal, subcutaneous, and peroral routes of administration. T. sinapou extract also inhibited formalin- and complete Freund’s adjuvant- (CFA-) induced flinching/licking at 100 mg/kg dose. In conclusion, these findings demonstrate that T. sinapou ethyl acetate extract reduces inflammatory pain in the acetic acid, PBQ, formalin, and CFA models of overt pain-like behavior. Therefore, the potential of analgesic activity of T. sinapou indicates that it deserves further investigation.
