Effect of Kamistad gel on oral ulcer healing and TNF ⁃ α, IL ⁃ 6 and EGF expression in ulcer tissue of rat

Abstract

Objective To investigate the effect of Kamistad gel on oral ulcer healing and the expression of tumor necrosis factor α (TNF⁃α), interleukin 6 (IL⁃6) and epidermal growth factor (EGF) after oral administration in ulcer tis⁃ sue of rats and to provide animal experimental data for the clinical application of Kamistad gel. Methods The oral ul⁃ cer rat model was established by chemical cauterization. The rats were randomly divided into four groups: Kamistad group (Kamistad gel), befuxin group (befuxin gel), lidocaine group (lidocaine cream), blank control group (normal sa⁃ line), with 12 rats in each group. The ulcer area of the rats in each group was measured before and 1, 3 and 5 days after treatment; at 1 day after treatment, the duration of swabbing behavior within 3 minutes of intraoral capsaicin infusion was recorded to evaluate the degree of pain; the ulcer tissue was collected at 5 days after treatment, and the histopatho⁃ logical changes were observed by HE staining, the expression of TNF⁃α, IL⁃6 and EGF in the ulcer tissue was detected by immunohistochemistry and ELISA. Results At 1 day after treatment, the duration of mouth wiping induced by cap⁃ saicin was significantly shorter in the Kamistad group than in the blank control and befuxin groups (P 0.05). At 5 days after treatment, the ulcer area was significantly smaller in the Kamistad group than in the blank control and lidocaine groups (P 0.05). At 5 days after treatment, H&E staining of the oral ulcer tissue sections showed significantly reduced levels of inflammatory cells and significantly pro⁃ liferated fibroblasts and better epithelial hyperplasia in the Kamistad group compared with those in the lidocaine and blank control groups, and there were no differences between the Kamistad and befuxin groups. At 5 days after treat⁃ ment, the expression levels of TNF⁃α, IL⁃6 and EGF in the ulcer tissue of rats in each group were significantly different (P 0.05). Con⁃ clusion Kamistad gel exhibited anti⁃inflammatory, analgesic and healing effects on experimental oral ulcers.

Keywords

• Oral ulcer
• Kamistad gel
• Rat
• Tumor necrosis factor⁃α
• Interleukin⁃6
• Epidermal growth factor