Effect of cadaverine on Brassica juncea (RH-30) seedlings under multiple stress - A quantitative analysis

Abstract

Background: Plants, in general, are subject to various kinds of stress, biotic and abiotic, both natural and manmade. Cadaverine (Cad) is a lysine catabolite and is involved in the growth and development of plants. The present study aimed to determine the effects of cadaverine response on the induction of polypeptide profile under multiple stresses in leaf and root tissues of Brassica juncea, and to diagnose the changes in gene expression (if any). The protein content was analyzed using SDSPAGE and the results were further validated using densitometry. The basal medium used for tissue culture was MS medium, formulated with different stress conditions and treatments without and with Cad (100mM NaCl, 1mM Cd or Pb, 5mM NH4NO3, 1mM cadaverine). Cad treatment showed increased protein content in the presence of NH4NO3, even in seedlings provided/ supplemented with multiple stresses. The protein content was analyzed using SDS-PAGE and the results were further validated using densitometry. Supplementation of Cad induced the expression of 5 more peptides in leaf tissue, such that were not observed in a leaf of the seedling without Cad. Cad enrichment in root tissue did not change the expression of any peptide in NaCl environment, but it was affected in the absence of Cad by saline conditions. The activating performance of Cad in increasing the expression of peptides, and its mitigating the different effects of multiple stresses, suggest its role in growth and development of leaf and root tissues of Brassica juncea (RH-30).

Keywords

• brassica juncea
• cadaverine
• densitometry
• polyamines
• protein
• quantification
• stress