Frontiers in Nutrition (Oct 2021)
Skimmed Bovine Milk-Derived Extracellular Vesicles Isolated via “Salting-Out”: Characterizations and Potential Functions as Nanocarriers
- Xiao-Hui Tan,
- Xiao-Hui Tan,
- Xiao-Hui Tan,
- Dong Fang,
- Dong Fang,
- Dong Fang,
- Yong-De Xu,
- Tie-Gui Nan,
- Wen-Peng Song,
- Wen-Peng Song,
- Wen-Peng Song,
- Yang-Yang Gu,
- Yang-Yang Gu,
- Yang-Yang Gu,
- Sheng-Ji Gu,
- Sheng-Ji Gu,
- Sheng-Ji Gu,
- Yi-Ming Yuan,
- Yi-Ming Yuan,
- Yi-Ming Yuan,
- Zhong-Cheng Xin,
- Zhong-Cheng Xin,
- Zhong-Cheng Xin,
- Li-Qun Zhou,
- Li-Qun Zhou,
- Li-Qun Zhou,
- Rui-Li Guan,
- Rui-Li Guan,
- Rui-Li Guan,
- Xue-Song Li,
- Xue-Song Li,
- Xue-Song Li
Affiliations
- Xiao-Hui Tan
- Department of Urology, Peking University First Hospital, Beijing, China
- Xiao-Hui Tan
- Institute of Urology, Peking University, Beijing, China
- Xiao-Hui Tan
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Dong Fang
- Department of Urology, Peking University First Hospital, Beijing, China
- Dong Fang
- Institute of Urology, Peking University, Beijing, China
- Dong Fang
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Yong-De Xu
- Department of Urology, Beijing Friendship Hospital, Capital Medical University, Beijing, China
- Tie-Gui Nan
- State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
- Wen-Peng Song
- Institute of Urology, Peking University, Beijing, China
- Wen-Peng Song
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Wen-Peng Song
- Department of Dental Implant Center, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, China
- Yang-Yang Gu
- Institute of Urology, Peking University, Beijing, China
- Yang-Yang Gu
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Yang-Yang Gu
- Department of Radiation Medicine, Institute of Systems Biomedicine, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China
- Sheng-Ji Gu
- Department of Urology, Peking University First Hospital, Beijing, China
- Sheng-Ji Gu
- Institute of Urology, Peking University, Beijing, China
- Sheng-Ji Gu
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Yi-Ming Yuan
- Department of Urology, Peking University First Hospital, Beijing, China
- Yi-Ming Yuan
- Institute of Urology, Peking University, Beijing, China
- Yi-Ming Yuan
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Zhong-Cheng Xin
- Department of Urology, Peking University First Hospital, Beijing, China
- Zhong-Cheng Xin
- Institute of Urology, Peking University, Beijing, China
- Zhong-Cheng Xin
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Li-Qun Zhou
- Department of Urology, Peking University First Hospital, Beijing, China
- Li-Qun Zhou
- Institute of Urology, Peking University, Beijing, China
- Li-Qun Zhou
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Rui-Li Guan
- Department of Urology, Peking University First Hospital, Beijing, China
- Rui-Li Guan
- Institute of Urology, Peking University, Beijing, China
- Rui-Li Guan
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- Xue-Song Li
- Department of Urology, Peking University First Hospital, Beijing, China
- Xue-Song Li
- Institute of Urology, Peking University, Beijing, China
- Xue-Song Li
- Beijing Key Laboratory of Urogenital Diseases (male) Molecular Diagnosis and Treatment Center, Beijing, China
- DOI
- https://doi.org/10.3389/fnut.2021.769223
- Journal volume & issue
-
Vol. 8
Abstract
Bovine milk-derived extracellular vesicles (BM-EVs) are recognized as promising nanoscale delivery vectors owing to their large availability. However, few isolation methods can achieve high purity and yield simultaneously. Therefore, we developed a novel and cost-effective procedure to separate BM-EVs via “salting-out.” First, BM-EVs were isolated from skimmed milk using ammonium sulfate. The majority of BM-EVs were precipitated between 30 and 40% saturation and 34% had a relatively augmented purity. The separated BM-EVs showed a spherical shape with a diameter of 60–150 nm and expressed the marker proteins CD63, TSG101, and Hsp70. The purity and yield were comparable to the BM-EVs isolated via ultracentrifugation while ExoQuick failed to separate a relatively pure fraction of BM-EVs. The uptake of BM-EVs into endothelial cells was dose- and time-dependent without significant cytotoxicity. The levels of endothelial nitric oxide syntheses were regulated by BM-EVs loaded with icariside II and miRNA-155-5p, suggesting their functions as delivery vehicles. These findings have demonstrated that it is an efficient procedure to isolate BM-EVs via “salting-out,” holding great promise toward therapeutic applications.
Keywords
