Castration modulates singing patterns and electrophysiological properties of RA projection neurons in adult male zebra finches
Songhua Wang,
Congshu Liao,
Fengling Li,
Shaoyi Liu,
Wei Meng,
Dongfeng Li
Affiliations
Songhua Wang
School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou, China
Congshu Liao
School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou, China
Fengling Li
School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou, China
Shaoyi Liu
School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou, China
Wei Meng
School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou, China
Dongfeng Li
School of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Higher Education of Guangdong Province, Guangzhou, China
Castration can change levels of plasma testosterone. Androgens such as testosterone play an important role in stabilizing birdsong. The robust nucleus of the arcopallium (RA) is an important premotor nucleus critical for singing. In this study, we investigated the effect of castration on singing patterns and electrophysiological properties of projection neurons (PNs) in the RA of adult male zebra finches. Adult male zebra finches were castrated and the changes in bird song assessed. We also recorded the electrophysiological changes from RA PNs using patch clamp recording. We found that the plasma levels of testosterone were significantly decreased, song syllable’s entropy was increased and the similarity of motif was decreased after castration. Spontaneous and evoked firing rates, membrane time constants, and membrane capacitance of RA PNs in the castration group were lower than those of the control and the sham groups. Afterhyperpolarization AHP time to peak of spontaneous action potential (AP) was prolonged after castration.These findings suggest that castration decreases song stereotypy and excitability of RA PNs in male zebra finches.
