Enhanced exercise and regenerative capacity in a mouse model that violates size constraints of oxidative muscle fibres
Saleh Omairi,
Antonios Matsakas,
Hans Degens,
Oliver Kretz,
Kenth-Arne Hansson,
Andreas Våvang Solbrå,
Jo C Bruusgaard,
Barbara Joch,
Roberta Sartori,
Natasa Giallourou,
Robert Mitchell,
Henry Collins-Hooper,
Keith Foster,
Arja Pasternack,
Olli Ritvos,
Marco Sandri,
Vihang Narkar,
Jonathan R Swann,
Tobias B Huber,
Ketan Patel
Affiliations
Saleh Omairi
School of Biological Sciences, University of Reading, Reading, United Kingdom
Antonios Matsakas
Hull York Medical School, Hull, United Kingdom
Hans Degens
School of Healthcare Science, Manchester Metropolitan University, Manchester, United Kingdom; Lithuanian Sports University, Kaunas, Lithuania
Oliver Kretz
Renal Division, University Medical Center Freiburg, Freiburg, Germany; Faculty of Medicine, University of Freiburg, Freiburg, Germany
Kenth-Arne Hansson
Centre for Integrative Neuroplasticity, Department of Biosciences, University of Oslo, Oslo, Norway
Andreas Våvang Solbrå
Centre for Integrative Neuroplasticity, Department of Biosciences, University of Oslo, Oslo, Norway; Department of Physics, University of Oslo, Oslo, Norway
Jo C Bruusgaard
Centre for Integrative Neuroplasticity, Department of Biosciences, University of Oslo, Oslo, Norway; Department of Health Sciences, Kristiania University College, Oslo, Norway
Barbara Joch
Department of Neuroanatomy, University of Freiburg, Freiburg, Germany; Faculty of Medicine, University of Freiburg, Freiburg, Germany
Roberta Sartori
Venetian Institute of Molecular Medicine, University of Padua, Padua, Italy
Natasa Giallourou
Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom
Robert Mitchell
School of Biological Sciences, University of Reading, Reading, United Kingdom
Henry Collins-Hooper
School of Biological Sciences, University of Reading, Reading, United Kingdom
Keith Foster
School of Biological Sciences, University of Reading, Reading, United Kingdom
Arja Pasternack
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Olli Ritvos
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland
Marco Sandri
Venetian Institute of Molecular Medicine, University of Padua, Padua, Italy
Vihang Narkar
Institute of Molecular Medicine, University of Health Science Center, Houston, Texas
Jonathan R Swann
Department of Food and Nutritional Sciences, University of Reading, Reading, United Kingdom
Tobias B Huber
Renal Division, University Medical Center Freiburg, Freiburg, Germany; Faculty of Medicine, University of Freiburg, Freiburg, Germany; BIOSS Center for Biological Signalling Studies, Albert-Ludwigs-University Freiburg, Houston, Texas; FRIAS, Freiburg Institute for Advanced Studies and Center for Biological System Analysis ZBSA, Freiburg, Germany
School of Biological Sciences, University of Reading, Reading, United Kingdom; FRIAS, Freiburg Institute for Advanced Studies and Center for Biological System Analysis ZBSA, Freiburg, Germany
A central tenet of skeletal muscle biology is the existence of an inverse relationship between the oxidative fibre capacity and its size. However, robustness of this relationship is unknown. We show that superimposition of Estrogen-related receptor gamma (Errγ) on the myostatin (Mtn) mouse null background (Mtn-/-/ErrγTg/+) results in hypertrophic muscle with a high oxidative capacity thus violating the inverse relationship between fibre size and oxidative capacity. We also examined the canonical view that oxidative muscle phenotype positively correlate with Satellite cell number, the resident stem cells of skeletal muscle. Surprisingly, hypertrophic fibres from Mtn-/-/ErrγTg/+ mouse showed satellite cell deficit which unexpectedly did not affect muscle regeneration. These observations 1) challenge the concept of a constraint between fibre size and oxidative capacity and 2) indicate the important role of the microcirculation in the regenerative capacity of a muscle even when satellite cell numbers are reduced.
