A Review for In Vitro and In Vivo Detection and Imaging of Gaseous Signal Molecule Carbon Monoxide by Fluorescent Probes

Can Xie; Kun Luo; Libin Tan; Qiaomei Yang; Xiongjie Zhao; Liyi Zhou

doi:10.3390/molecules27248842

Molecules (Dec 2022)

A Review for In Vitro and In Vivo Detection and Imaging of Gaseous Signal Molecule Carbon Monoxide by Fluorescent Probes

Can Xie,
Kun Luo,
Libin Tan,
Qiaomei Yang,
Xiongjie Zhao,
Liyi Zhou

Affiliations

Can Xie: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Kun Luo: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Libin Tan: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Qiaomei Yang: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Xiongjie Zhao: College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China
Liyi Zhou: College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China

DOI: https://doi.org/10.3390/molecules27248842
Journal volume & issue: Vol. 27, no. 24
p. 8842

Abstract

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Carbon monoxide (CO) is a vital endogenous gaseous transmitter molecule involved in the regulation of various physiological and pathological processes in living biosystems. In order to investigate the biological function of CO, many technologies have been developed to monitor the level of endogenous CO in biosystems. Among them, the fluorescence detection technology based on the fluorescent probe has the advantages of high sensitivity, excellent selectivity, simple operation, especially non-invasive damage to biological samples, and the possibility of real-time in situ detection, etc., which is considered to be one of the most effective and applicable detection techniques. Therefore, in the last few years, a lot of work has been carried out on the design, synthesis and in vivo fluorescence imaging studies of CO fluorescent probes. Furthermore, using fluorescent probes to detect the changes in CO concentrations in living cells and tissues as well as in organisms has been one of the hot research topics in recent years. However, it is still a challenge to rationally design CO fluorescent probe with excellent optical performance, structural stability, low background interference, good biocompatibility, and excellent water solubility. Therefore, this review focuses on the research progress of CO fluorescent probes in the detection mechanism and biological applications in recent years. However, this popular and leading topic has rarely been summarized comprehensively to date. Thus, the research progress of CO fluorescent probes in recent years is reviewed in terms of their design concept, detection mechanism, and their biological applications. In addition, the relationship between the structure and performance of the probes was also discussed. More significantly, we hope that more excellent optical properties fluorescent probes for gaseous transmitter molecule CO detection and imaging will overcome the current problems of high biotoxicity and limited water solubility in future.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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