Nature Communications (Jul 2022)
A metagenomic DNA sequencing assay that is robust against environmental DNA contamination
- Omary Mzava,
- Alexandre Pellan Cheng,
- Adrienne Chang,
- Sami Smalling,
- Liz-Audrey Kounatse Djomnang,
- Joan Sesing Lenz,
- Randy Longman,
- Amy Steadman,
- Luis G. Gómez-Escobar,
- Edward J. Schenck,
- Mirella Salvatore,
- Michael J. Satlin,
- Manikkam Suthanthiran,
- John R. Lee,
- Christopher E. Mason,
- Darshana Dadhania,
- Iwijn De Vlaminck
Affiliations
- Omary Mzava
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- Alexandre Pellan Cheng
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- Adrienne Chang
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- Sami Smalling
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- Liz-Audrey Kounatse Djomnang
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- Joan Sesing Lenz
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- Randy Longman
- Division of Gastroenterology and Hepatology, Weill Cornell Medicine, Jill Roberts Center for IBD
- Amy Steadman
- Global Health Labs
- Luis G. Gómez-Escobar
- Division of Pulmonary and Critical Care Medicine, Department of Medicine, Weill Cornell Medicine
- Edward J. Schenck
- Division of Pulmonary and Critical Care Medicine, Department of Medicine, Weill Cornell Medicine
- Mirella Salvatore
- Divisionof Public Health Programs, Department of Medicine, Weill Cornell Medicine
- Michael J. Satlin
- Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine
- Manikkam Suthanthiran
- Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medicine
- John R. Lee
- Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medicine
- Christopher E. Mason
- Department of Physiology and Biophysics, Weill Cornell Medical College
- Darshana Dadhania
- Division of Nephrology and Hypertension, Department of Medicine, Weill Cornell Medicine
- Iwijn De Vlaminck
- Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University
- DOI
- https://doi.org/10.1038/s41467-022-31654-0
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 10
Abstract
The accuracy of metagenomic DNA sequencing is limited by environmental DNA contamination. Here, the authors develop and test SIFT-seq, a metagenomic DNA sequencing assay that allows to identify and remove environmental DNA contamination introduced during sample preparation.
