Surface Functionalization of SBA-15 for Immobilization of Myoglobin

Hengmin Miao; Hengmin Miao; Maosheng Li; Maosheng Li; Fang Wang; Jiao Li; Ying-Wu Lin; Ying-Wu Lin; Jiakun Xu

doi:10.3389/fbioe.2022.907855

Frontiers in Bioengineering and Biotechnology (May 2022)

Surface Functionalization of SBA-15 for Immobilization of Myoglobin

Hengmin Miao,
Hengmin Miao,
Maosheng Li,
Maosheng Li,
Fang Wang,
Jiao Li,
Ying-Wu Lin,
Ying-Wu Lin,
Jiakun Xu

Affiliations

Hengmin Miao: Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao, China
Hengmin Miao: School of Materials Science and Engineering, Shandong University of Technology, Zibo, China
Maosheng Li: Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao, China
Maosheng Li: School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
Fang Wang: Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao, China
Jiao Li: School of Materials Science and Engineering, Shandong University of Technology, Zibo, China
Ying-Wu Lin: School of Chemistry and Chemical Engineering, University of South China, Hengyang, China
Ying-Wu Lin: Key Laboratory of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang, China
Jiakun Xu: Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao, China

DOI: https://doi.org/10.3389/fbioe.2022.907855
Journal volume & issue: Vol. 10

Abstract

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Mesoporous molecular sieve SBA-15 was successfully modified with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The functionalized SBA-15 were characterized by small-angle X-ray (SAXRD), thermogravimetric analysis (TG), N2 adsorption, and Fourier transformed infrared spectrum (FT-IR). APTES functionalized SBA-15 (named SBA-15-A) and GPTMS functionalized SBA-15 (named SBA-15-G) were used to immobilize myoglobin (Mb). The loading amounts of Mb by SBA-15-A and SBA-15-G were 511.2 and 547.8 mg/g, respectively, whereas only 359.6 mg/g was achieved by SBA-15. Mb/SBA-15-G and Mb/SBA-15-A demonstrated better reusability than SBA-15, retaining 84.6% and 82.7% of the initial activity after repeated use seven times. The Mb/SBA-15-A and Mb/SBA-15-G also exhibited improved thermal stability and storage stability.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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