Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences

Amanda R. Krysler; Christopher R. Cromwell; Tommy Tu; Juan Jovel; Basil P. Hubbard

doi:10.1038/s41467-022-29202-x

Nature Communications (Mar 2022)

Guide RNAs containing universal bases enable Cas9/Cas12a recognition of polymorphic sequences

Amanda R. Krysler,
Christopher R. Cromwell,
Tommy Tu,
Juan Jovel,
Basil P. Hubbard

Affiliations

Amanda R. Krysler: Department of Pharmacology, University of Alberta
Christopher R. Cromwell: Department of Pharmacology, University of Alberta
Tommy Tu: Department of Pharmacology, University of Alberta
Juan Jovel: The Applied Genomics Core, Office of Research, University of Alberta
Basil P. Hubbard: Department of Pharmacology, University of Alberta

DOI: https://doi.org/10.1038/s41467-022-29202-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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Genetic variance poses a major challenge for CRISPR-based human therapeutics and pathogen diagnostics. Here the authors show how to circumvent this issue by using guide RNAs containing universal bases to tailor Cas9/Cas12 specificity.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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