Datura metel L. inhibits the secretion of inflammatory factors and angiogenesis in keratinocytes in vitro

Abstract

Objective To investigate the potential role of the traditional herbal medicine Datura metel L. in the treatment of psoriasis using TNF-α-induced inflammation in keratinocytes as a model. Methods Keratinocyte cell line HaCaT was used to establish a psoriasis cell model by tumor necrosis factor-alpha (TNF-α) treatment. The experiment comprised three groups: a blank control group, TNF-α-induced psoriasis model group, and TNF-α + Datura metel L. intervention group. Level of IL-17 and CCL20 was measured ELISA, expression of nuclear factor kappa B (NF-κB) subunit p65 protein was measured by Western blot. Endothelial cell tube formation experiment was conducted using an in vitro angiogenesis analysis kit. Results Compared to the TNF-α-induced psoriasis model group, the Datura metel L. extract significantly reduced the levels of IL-17 and CCL20 in the cell culture supernatant of TNF-α-induced psoriasis model (P＜0.001); Datura metel L. extract markedly decreased the NF-κB subunit p65 protein level in TNF-α-induced psoriasis model cells (P＜0.01); Datura metel L. extract effectively inhibited the induction of endothelial cell tube formation by the cell culture supernatant of the psoriasis model group. Conclusions The Datura metel L. extract down-regulates NF-κB signaling pathway molecules, reducing the production of IL-17 and CCL20 inflammatory factors in inflammatory keratinocytes and inhibiting angiogenesis.

Keywords

• psoriasis|datura metel l.|ccl20|angiogenesis