Cuticular damage of Lucilia cuprina larvae exposed to Curcuma longa leaves essential oil and its major compound α-phellandrene
Amanda Chaaban,
Vinicius Sobrinho Richardi,
Alessandra Regina Carrer,
Juliana Sperotto Brum,
Roger Raupp Cipriano,
Carlos Eduardo Nogueira Martins,
Mário Antônio Navarro-Silva,
Cicero Deschamps,
Marcelo Beltrão Molento
Affiliations
Amanda Chaaban
Laboratory of Parasitic Diseases, Department of Veterinary Sciences, Federal University of Parana, UFPR, Curitiba, PR, Brazil; Department of Veterinary Medicine, Catarinense Federal Institute, IFC, Araquari, SC, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil; Corresponding author at: Laboratory of Parasitic Diseases, Department of Veterinary Sciences, Federal University of Parana, UFPR, Curitiba, PR, Brazil.
Vinicius Sobrinho Richardi
Laboratory of Morphology and Physiology of Culicidae and Chironomidae, Federal University of Parana, UFPR, Curitiba, PR, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Alessandra Regina Carrer
Department of Veterinary Medicine, Catarinense Federal Institute, IFC, Araquari, SC, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Juliana Sperotto Brum
Laboratory of Veterinary Pathology, Department of Veterinary Sciences, Federal University of Parana, Curitiba, PR, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Roger Raupp Cipriano
Laboratory of Phytotech and Crop Protection, Federal University of Paraná, UFPR, Curitiba, PR, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Carlos Eduardo Nogueira Martins
Department of Veterinary Medicine, Catarinense Federal Institute, IFC, Araquari, SC, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Mário Antônio Navarro-Silva
Laboratory of Morphology and Physiology of Culicidae and Chironomidae, Federal University of Parana, UFPR, Curitiba, PR, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Cicero Deschamps
Laboratory of Phytotech and Crop Protection, Federal University of Paraná, UFPR, Curitiba, PR, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Marcelo Beltrão Molento
Laboratory of Parasitic Diseases, Department of Veterinary Sciences, Federal University of Parana, UFPR, Curitiba, PR, Brazil; Laboratory of Veterinary Pathology, Department of Veterinary Sciences, Federal University of Parana, Curitiba, PR, Brazil; Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil
Morphological biomarkers as the histopathological assessment and scanning electron microscopy can be used to establish a diagnosis of structure damage and intoxication of target cells by new biopesticide candidate. In this sense, cuticle damage caused by active substances in larvae exposed to biopesticides can help to elucidate the mode action. Thus, insecticide activity analysis of essential oil of Curcuma longa leaves and its major compound α-phellandrene have proven to be a new biopesticide candidate against third instar larvae (L3) of the Australian blowfly Lucilia cuprina. In this way, groups of 20 L3 were placed on filter paper, impregnated with ranging concentrations (from 0.15 to 2.86 μL/cm2) of C. longa leaves EO and (0.29–1.47 μL/cm2) to α-phellandrene. The extracts were solubilized in ethanol. Progressive darkening in the body of L3, marked reduction of movement, color changes in larval cuticle and dead were observed 6 and 24 h after contact with both extracts.
