Karakterisasi Sifat Ketahanan Terhadap Naungan pada Kedelai dengan Marka RAPD

Abstract

A series of experiment of soybean tolerance to shade were conducted in order to attain the following objectives: genotypes evaluation for shade tolerance, appropriate selection method to screen tolerant genotype to shade characterization of molecular markers of shade tolerance by using RAPD marker. The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. Molecular analysis by using RAPD technique showed that UBC 153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for linkage analysis. All polymorphisms segregated independently of each ather. The nearest genetic distance was DNA fregment about 125 bp of UBC 153 marker which is linked to tolerance to shade character by about 0.13 centomorgans.

Keywords

• kedelai
• Glycine max (L.) Merrill
• tolerance
• shade
• RAPD