Main Polar Metabolites from Leaves of the Native Andean Species Jungia rugosa Less (Asteraceae)

Abstract

Abstract The ethanolic extract of Jungia rugosa Less, a popular Andean species belonging to the family Asteraceae, was characterized chemically. The extract was deprived of chlorophyll by solid-phase extraction, using Diaion Hp-20 resin as solid phase, and eluting with a mixture of EtOH/H 2 O according to a decreasing polarity gradient. The chlorophyll-free extract was then repeatedly fractionated by open column chromatography in normal phase and preparative thin layer chromatography. Two main metabolites were finally purified and identified through structure elucidation. Nuclear magnetic resonance spectroscopy (NMR) and electrospray ionization-mass spectrometry (ESI-MS) were the 2 techniques used for analysis. The identified metabolites were coumarins: (1) umbelliferone and (2) coumarin-5-methyl-4α-glucoside. Compound 1 is known in the literature for having anti-inflammatory, antihyperglycemic, and antitumor activities. Compound 2 has not been described before for this botanical genus and its pharmacological effects are still uninvestigated.

Keywords

• jungia rugosa, umbelliferone, coumarin-5-methyl-4 α -glucoside, nmr, esi-ms, ecuador.