Frontiers in Microbiology (Dec 2020)

Extended-Spectrum β-Lactamases in Human Isolates of Multidrug-Resistant Non-typhoidal Salmonella enterica

  • Anahit M. Sedrakyan,
  • Zhanna A. Ktsoyan,
  • Karine A. Arakelova,
  • Magdalina K. Zakharyan,
  • Alvard I. Hovhannisyan,
  • Zaruhi U. Gevorgyan,
  • Armine A. Mnatsakanyan,
  • Elene G. Kakabadze,
  • Elene G. Kakabadze,
  • Khatuna B. Makalatia,
  • Khatuna B. Makalatia,
  • Nina A. Chanishvili,
  • Jean-Paul Pirnay,
  • Arsen A. Arakelyan,
  • Rustam I. Aminov,
  • Rustam I. Aminov

DOI
https://doi.org/10.3389/fmicb.2020.592223
Journal volume & issue
Vol. 11

Abstract

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A total of 291 non-duplicate isolates of non-typhoidal Salmonella (NTS) were collected from the fecal samples of patients with salmonellosis in Armenia and Georgia during 1996–2016. The isolates were tested for resistance to antimicrobials, including extended-spectrum β-lactamases (ESBL). The high prevalence of multidrug-resistance (MDR) and ESBL-producer phenotypes was detected among Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) isolates collected from patients in Armenia between 1996 and 2016. A total of 36 MDR NTS isolates were subjected to whole genome sequencing (WGS) to determine the genetic background of antimicrobial resistance (AMR) and mobile genetic elements. All ESBL-producing S. Typhimurium isolates belonged to the same sequence type (ST328). The ESBL-producer phenotype was associated with plasmid-encoded CTX-M-5 production. A range of other plasmids was associated with resistance to other antimicrobials, including the MDR phenotype.

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