Armaghane Danesh Bimonthly Journal (Dec 2019)
The Effect of 14 Weeks of Endurance Training on MYH7 and MYH7β Gene Expression and Left Ventricular Structural Changes in Male Wistar Rats
Abstract
Background & aim: MYH7 and MYH7β genes play important roles in cardiac function. Endurance activity, on the other hand, challenges the structure and function of the heart. The aim of the present study was to determine and evaluate the effect of 14 weeks of endurance training on MYH7 and MYH7β gene expression and left ventricular structural changes in male Wistar rats. Methods: In the present experimental research, 14 rats weighing 113±20gr under controlled conditions (temperature, light and dark cycle, free access to food and water) were retained. After exposure (231±24g), they were randomly divided into control (7) and experimental (7) groups. The experimental group completed a program (30 m/min, 50 min/session, 6 sessions/week for 14 weeks) on the treadmill and then anesthetized and described 48 hours after the last training session with the control group. Then their hearts and their left ventricles were removed and MYH7 and MYH7β gene expression levels were measured using Real time-PCR. Data were analyzed using independent and dependent t-tests. Results: The results of the present study indicated that cardiac weight gain and MYH7 and MYH7β gene expression were increased in the exercise group, meaning that endurance exercise significantly increased the heart to body weight ratio in the experimental group compared to the control group. Expression of MYH7 and MYH7β genes was not significantly higher in the experimental group (p = 0.005) (p = 0.001). Conclusion: It seemed that the endurance training induced remodeling which was indicated by hypertrophy and it coincided with increasing of MYH7 and MYH7b genes expression. This may be part of an improvement in cardiac function due to exercise activity due to increased expression of these genes
