Intensification of extraction process through IVDV pretreatment from Eryngium creticum leaves and stems: Maximizing yields and assessing biological activities
Mariam Hammoud,
Espérance Debs,
Lambertus A.M. van den Broek,
Hiba N. Rajha,
Carl Safi,
Gijs van Erven,
Richard G. Maroun,
Ali Chokr,
Hassan Rammal,
Nicolas Louka
Affiliations
Mariam Hammoud
Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-alimentaire, Faculté des Sciences, Université Saint-Joseph, B.P. 17-5208 Riad El Solh, Beirut, Lebanon; Research Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, Beirut, Lebanon; Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technology, Lebanese University, Hadath Campus, Beirut, Lebanon
Espérance Debs
Department of Biology, Faculty of Arts and Sciences, University of Balamand, Tripoli P. O. Box 100, Lebanon
Lambertus A.M. van den Broek
Wageningen Food & Biobased Research, PO Box 17, 6700 AA Wageningen, the Netherlands; Corresponding author.
Hiba N. Rajha
Ecole Supérieure d’Ingénieurs de Beyrouth (ESIB), Saint-Joseph University, Mkalles Mar Roukos, Beirut, Lebanon
Carl Safi
Wageningen Food & Biobased Research, PO Box 17, 6700 AA Wageningen, the Netherlands
Gijs van Erven
Wageningen Food & Biobased Research, PO Box 17, 6700 AA Wageningen, the Netherlands
Richard G. Maroun
Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-alimentaire, Faculté des Sciences, Université Saint-Joseph, B.P. 17-5208 Riad El Solh, Beirut, Lebanon
Ali Chokr
Research Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, Beirut, Lebanon; Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technology, Lebanese University, Hadath Campus, Beirut, Lebanon
Hassan Rammal
Research Laboratory of Microbiology (RLM), Department of Life and Earth Sciences, Faculty of Sciences I, Lebanese University, Hadath Campus, Beirut, Lebanon; Platform of Research and Analysis in Environmental Sciences (PRASE), Doctoral School of Sciences and Technology, Lebanese University, Hadath Campus, Beirut, Lebanon; Faculty of Agronomy, Lebanese University, Dekweneh-Lebanon
Nicolas Louka
Centre d’Analyses et de Recherche, Unité de Recherche Technologies et Valorisation Agro-alimentaire, Faculté des Sciences, Université Saint-Joseph, B.P. 17-5208 Riad El Solh, Beirut, Lebanon
“Intensification of Vaporization by Decompression to the Vacuum” (IVDV) has initially emerged as a technology primarily employed for expanding and enhancing the texture of biological products. However, its recent applications have showcased significant promise in the realm of extracting bioactive molecules from various plant materials. In this context, optimization using response surface methodology was conducted to investigate the impact of IVDV pretreatment on the extractability of phenolic compounds from Eryngium creticum leaves and stems, as well as their biological activities. Using IVDV preceding the extraction led to higher total phenolic content (TPC) and enhanced antiradical activities in treated materials compared to untreated ones. The optimal processing conditions in terms of water content, steam pressure and treatment time were determined in order to maximize TPC (89.07 and 20.06 mg GAE/g DM in leaves and stems, respectively) and antiradical (DPPH) inhibition percentage (93.51% and 27.54% in leaves and stems, respectively). IVDV-treated extracts showed superior antioxidant, antibacterial and antibiofilm capacities compared to raw extracts. Using RP-UHPLC-PDA-MS, caffeic acid and rosmarinic acid were identified in IVDV-treated leaves. IVDV can be implemented as an innovative treatment applied prior to extraction to boost the recovery of biomolecules from plant matrices.
