Evaluation of Platelet Rich Fibrin Content Among Individuals With Different Blood Groups

Abstract

Objectives: Platelet Rich Fibrin (PRF), obtained by centrifuging the peripheral blood sample of the person is an autogenous material and can be affected by systemic and genetic characteristics of the belonging person. This study was undertaken for evaluating the effect of blood group types and the diverse preparation protocols on growth factors and cytokines contained in the PRF. Methods: In total, 192 blood samples were taken from 64 donors, including 16 individuals from each blood group of 4, and from each individual 3 samples were taken for obtaining PRF, titanium platelet‐rich fibrin (T-PRF), and CGF (concentrated growth factors). The platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), angiogenin (Ang), osteocalcin, and osteonectin contents of the PRF were measured with ELISA set. Results: When the relationship between blood groups was examined, the results indicated that there were no statistically significant differences (p>0.05). On the other hand, growth factor content was evaluated to be higher in T-PRF than PRF and CGF (p<0.05). Conclusion: These data clearly demonstrate that PRF, T-PRF and CGF preparations contain significant amounts of growth factors capable of stimulating wound healing. However, this content is independent of blood group distribution and depends on fibrin acquisition protocols. Keywords; Blood group antigens, Enzyme-linked immunosorbent assay, Fibroblast growth factors, Osteocalcin, platelet-rich fibrin,

Keywords

• blood group antigens
• enzyme-linked immunosorbent assay
• fibroblast growth factors
• osteocalcin
• platelet-rich fibrin